This Image shows the PARISS system mounted on a typical research quality microscope. In this configuration it is possible to use the system as an analytical “spectrometer on microscope,” or a Hyperspectral imaging system.Authors using PARISS have been awarded the front page of journals four times click here to view
The “PARISS Hyperspectral imaging system” schematic describing the imaging spectrograph module.The curved prism corrects aberrations to enable excellent spectral imaging. A Hyperspectral image of an unlimited, heterogeneous, field-of-view (FOV) can be generated by translating a FOV under computer control. Spectral information is acquired with a scientific CCD camera.
The image on the left shows a living cell that has internalized a gold and nickel nanowire.The image on the right shows a Hyperspectral image of the nanowire with the spectral reflection characteristics of both gold and nickel
This Hyperspectral image of a tissue section shows the spatial location of three different and highly spectrally overlapping fluorophores.The Hyperspectral image succeeded in segmenting, or un-mixing, the three fluorophores to show their location in the section.
A low pressure mercury/Argon calibration spectrum taken with the PARISS Hyperspectral Imaging System.All wavelengths were acquired simultaneously. Note that unlike a diffraction grating there is absolutely no second, or higher order contamination
Copyright © LightForm Inc, 2012
PARISS: Prism And Reflector Imaging Spectroscopy System
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LightForm Inc: PARISS® Analytical Spectral and Hyperspectral Imaging
Hyperspectral Imaging For Bio-Medical, Biological and Materials Science Use in:
Fluorescence, Absorption, %Reflection, %Transmission, Luminescence, Dark-Field Scatter
CCD spectrum
camera
Curved prism and
back reflector
Field of view translates
under computer control
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