|
|
|
PARISS® Analytical Hyperspectral Imaging Systems,
and Specifications
-
Interfaces
to all major upright and inverted microscopes as an
accessory
-
Patented PARISS imaging
spectrometer operates in "spectrograph" configuration with no moving parts. All wavelengths are acquired simultaneously
-
Uses two Q-Imaging digital cameras one for wavelength measurement on the
PARISS spectrometer; the other to capture an observed
image of the field of view (FOV). (Note the observed image
camera takes no part in the spectral imaging process,
it is only there for the convenience of the operator.)
-
-
Hyperspectral/multispectral
software package that integrates all spectroscopy, spectral
correlation and topographical imaging functions
PARISS: Spectral Imaging System interface
Interfaces
to all major upright and inverted microscopes as an
accessory
-
Patented PARISS imaging
spectrograph
-
Uses one Q-Imaging digital cameras for wavelength measurement on the PARISS
spectrometer
-
All spectroscopic functions including
absorption (in OD), reflection, fluorescence, luminescence,
color measurement
PARISS Imaging Spectrometer specifications (1)
- Physical
size:
230 x 70 x 60 mm
- Supported cameras:
Most digital cameras manufactured by Q-Imaging (2)
- Optical
geometry patented curved prism and reflector (3)
- Type:
Imaging flat field spectrograph
- Moving
parts in the imaging spectrometer:
none
- Spectral
range Nominally 360 to 920 nm limited by the camera (4)
- Spectral
resolution 1 nm FWHM at 436 nm with standard Retiga EXi. Spectral resolution
is limited by the size of the pixels in the camera.
(5)
- Spectral
accuracy ~0.3 nm over the range from 365
to 800 nm. Depends on the pixel size of the camera
- Slit
width:
25 micron for the best compromise for spectral and spatial
resolution and light throughput (6)
- Slit
height 5
mm
- Spatial
resolution ~0.6 micron at the sample with a 25 micron slit-width and 40x objective (7)
- Computer
operated stage 10 nm/step over
an unlimited FOV.
An entire slide can be automatically scanned from end
to end.
Selected PARISS imaging spectroscopy software
functions
- LabVIEW The
PARISS software is written entirely in LabVIEW
- Spectral classification Automatic and manual: "unmix" multiple fluorophores.
- Standard spectroscopy
functions including
absorption (in Optical Density (OD)), %reflection, %transmittance,
fluorescence, luminescence,
- Data manipulation: file subtraction, addition, division, multiplication,
CIE color measurement. Spectral data can be saved in ASCII and exported to third party programs.
- Topographical mapping (spectral imaging) the location
of any chosen spectrum or set of spectra can be mapped
onto a grayscale image of the FOV to a user selected tolerance
- Spectral histograms All selected classified spectra
that appear in a spectral image can be represented
in ratio format as spectral histograms for statistical representation
- Spatial Regions of
Interest (ROI) can be selected and repeatedly scanned over a user selected
period of time
- Spectral ROI User selects the wavelength range of interest. PARISS
always acquires ALL wavelengths even if few are user selected
- Hardware control The PARISS software operates and synchronizes all functions
including both cameras and microscope stage
- File saving Save in ASCII, tif and prs (tif with headers to enable
off-line wavelength identification in stored files)
- Calibration A wavelength calibration lamp (MIDL) is supplied with every instrument to verify that the instrument
is in optimum alignment, focus and wavelength accuracy.
- Radiometry: PARISS can be standardized to a NIST tracable radiometric lamp. All data is then presented as power versus wavelength
PARISS data processor
key specifications
- Windows XP professional
- >3.4 GHz processor
- Slot for a firewire card
- >=750mHz
Notes
(1) Specifications and hardware choices
may change without notice
(2) LightForm reserves the right to add or remove any camera
or manufacturer from its list
(3) Prism assembly is manufactured under a license to US
patent 5,127,728 owned by The Aerospace Corporation.
(4) The nominal spectral range from 365 to 920 NM is limited
by the QE of the camera and the size of the chip. These
specifications can change without notice.
(5) Spectral resolution is limited by the width of the slit,
and the size of pixels on the camera. For a tutorial on
the Optics of Spectroscopy that goes into the details of
spectral resolution and wavelength linearity click
here.
(6) Fixed slit widths can be selected prior to purchase.
LightForm deliberately made this design choice because adjustable
slits makes it difficult, if possible, to maintain spectral
libraries.
(7) Actual spatial resolution depends on pixel size and
scattering in the sample. Spatial resolution can be approximated
by dividing the slit width by the magnification of the objective.
| |
|
|
©
1990-2010 LightForm, Inc all rights reserved |
|
|
|
|