"Fluorescence
Hyperspectral Imaging"

Use hyperspectral imaging to unmix
multiple fluorophores tissue as well as
living and prepared cells.

"PARISS® is a hyperspectral imaging instrument that simply
attaches to the video port of a research microscope.

Alexa spectra Hyperspectral tissue image
Spectral emission profiles of Alexa 555, 569, and 594. Emission spectra of each fluorophore strongly overlaps the others.
Tissue section stained with Alexa 555, 569 and 594; all excited simultaneously at 488 nm. The distribution of each fluorophore is clearly segmented**.

Key Applications

  • Spectral "unmixing" algorithm: spectral waveform cross-correlation. Accommodates non-linear mixing
  • CFP/YFP FRET: with over 300 wavelength data points! A recent FRET paper included the surprising statement:

    Quote: “While the biological implication for such protease activity we observed in some dying cells remains to be explored, these examples demonstrate the power of spectral imaging to monitor FRET. None of the other FRET measurement methods, including sensitized emission, acceptor recovery after photo-bleaching or donor fluorescent lifetime imaging (FLIM), can differentiate such situations.”[1]

  • Segment and delineate: Up to 15 fluorophores or chromophores simultaneously in fluorescence, or brightfield

  • Nano-materials: Quantum Dots, wires, rods and particles

  • OLED: (Organic LED) defect characterization

    [1] "Detection of Real-Time Mitochondrial Caspase Activity, in Situ, in Live Cells", Yingpei Zhang, Catherine Haskins, Marisa Lopez-Cruzan, Jianhua Zhang, Victoria Centonze-Frohlich, Brian Herman; Microsc Microanal.; 10:442-8 (Aug 2004)

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* "Alexa Fluor(R)" and "Molecular Probes(TM)" are trademarks of Invitrogen/Molecular Probes
** Taken from "Abstract_FRET_FLIM_Spectral Imaging2" Click here for the pdf

fluorescence hyperspectral imaging