The PARISS® Fluorescence
Hyperspectral Imaging

"PARISS is a Hyperspectral Fluorescence Imaging
Instrument That Attaches to the Video Port of a
Research Microscope.

Spectral emission profiles of Alexa® 555, 569, and 594. Emission spectra of each fluorophore strongly overlaps the others.	Hyperspectral Fluorescence image of a Tissue section stained with Alexa® 555, 569 and 594. All fluorophores were excited simultaneously at 488 nm.

Key Applications for Fluorescence Hyperspectral Imaging

• Spectral "unmixing" algorithm: spectral waveform cross-correlation. Accommodates non-linear mixing
• CFP/YFP FRET: with over 300 wavelength data points! A recent FRET paper included the surprising statement:
  
  Quote: “While the biological implication for such protease activity we observed in some dying cells remains to be explored, these examples demonstrate the power of spectral imaging to monitor FRET. None of the other FRET measurement methods, including sensitized emission, acceptor recovery after photo-bleaching or donor fluorescent lifetime imaging (FLIM), can differentiate such situations.”[1]
  
  
• Segment and delineate: Up to 15 fluorophores or chromophores simultaneously in fluorescence, or brightfield
  
  
• Fluorescent Nano-materials: Quantum Dots, wires, rods and particles
  
  
• OLED: (Organic LED) using auto-fluorescence for defect characterization
  
  [1] "Detection of Real-Time Mitochondrial Caspase Activity, in Situ, in Live Cells", Yingpei Zhang, Catherine Haskins, Marisa Lopez-Cruzan, Jianhua Zhang, Victoria Centonze-Frohlich, Brian Herman; Microsc Microanal.; 10:442-8 (Aug 2004)

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* "Alexa Fluor(R)" and "Molecular Probes(TM)" are trademarks of Invitrogen/Molecular Probes
** Taken from "Abstract_FRET_FLIM_Spectral Imaging2" Click here for the pdf

This page describes the PARISS Hyperspectral Fluorescence Imaging System