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"Hyperspectral Imaging
on a Microscope"
Unmix multiple fluorophores, segment brightfield, characterize nanoparticles
"PARISS® ": "Prism
and Reflector Imaging Spectroscopy System"
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Spectral emission profiles of Alexa
555, 569, and
594. Emission
spectra of each fluorophore strongly overlaps the
others. |
Tissue section stained with Alexa 555, 569 and 594; all excited simultaneously at 488 nm.
The distribution of each fluorophore is clearly
segmented**. |
Key functions
- Spectral range 365 to 920 nm in a single shot
- Spectral "unmixing"
with auto-spectral libraries
- Characterize
CFP/YFP FRET with over 300 wavelength
data points!
- Conduct
pure spectral analysis (absorption, % reflection, %transmission)
on micro samples
- Generate
spectral histograms for statistical analysis of regions of interest or entire
fields
Key applications
- Segment
and delineate up to 15 fluorophores
simultaneously
- Characterize individual nanoparticles for size and shape.
- Characterize extended fields of nanoparticles using MIE scattering
- Characterize nanorods and nanowires
- FRET and multiplexed fluorophore imaging
- Advanced segmentation and analysis of bright-field histological
samples
- OLED defect characterization
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©
1990-2008 LightForm, Inc all rights reserved |
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* "Alexa Fluor(R)"
and "Molecular Probes(TM)" are trademarks of
Invitrogen/Molecular Probes
** Taken from "Abstract_FRET_FLIM_Spectral Imaging2"
Click
here for the pdf
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LightForm, Inc.,
601 Route 206, Suite 26-479
Hillsborough NJ 08844
Tel: (908)281 9098
Email: jlerner@lightforminc.com
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