"Hyperspectral Imaging
For Life and Materials Science"
PARISS® Hyperspectral Imaging works in:
Fluorescence, Absorption, Reflection and Emission
"Unmixes" Multiple Fluorophores,
Segments Brightfield, and Will
Characterize and Detect Nanoparticles
The PARISS system is a microscope accessory
( PARISS: "Prism
and Reflector Imaging Spectroscopy System")
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| Spectral emission profiles of Alexa 555, 569, and
594. Emission
spectra of each fluorophore strongly overlaps the
others. |
Tissue section stained with Alexa 555, 569 and 594; all excited simultaneously at 488 nm.
The distribution of each fluorophore is clearly
segmented**. |
| Key Functions |
- Spectral range 365 to 920 nm in a single shot
- Spectral "unmixing" with auto-spectral libraries
- Characterize
CFP/YFP FRET with over 300 wavelength
data points!
- Conduct
pure spectral analysis (absorption, % reflection, %transmission)
on micro samples
- Generate spectral histograms for statistical analysis of regions of interest or entire
fields
- Radiometric calibration to compensate for variations in camera sensitivity (QE), as a function of wavelength
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| Key applications |
- Fluorescence Segmentation: delineate up to 15 fluorophores
simultaneously
- Nanoparticle, Nanowire and Nanorod characterization: Characterize individual nanoparticles for size and shape.
- Dark-Field Scatter: Characterize extended fields of nanoparticles using dark-field scatter
- FRET and multiplexed fluorophore imaging
- Advanced segmentation and analysis of bright-field histological
samples
- OLED defect characterization
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To play the PARISS Hyperspectral Imaging "animated" presentation
Click Here |
* "Alexa Fluor(R)"
and "Molecular Probes(TM)" are trademarks of
Invitrogen/Molecular Probes
** Taken from "Abstract_FRET_FLIM_Spectral Imaging2" Click
here for the pdf
PARISS®, MIDL® and Sylph(tm) are trademarks of LightForm, Inc.
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©
1990-2010 LightForm, Inc., all rights reserved |
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