Header

"Hyperspectral Imaging
In the Life and Materials Sciences"

Use Hyperspectral Imaging to:
"Unmix" Multiple Fluorophores,
Segment Brightfield,
Characterize and Detect Nanoparticles

The PARISS system is a microscope accessory

( PARISS: "Prism and Reflector Imaging Spectroscopy System")

 
hyperspectral imaging: Alexa spectra
hyperspectral imaging: Alexa in tissue
Spectral emission profiles of Alexa 555, 569, and 594. Emission spectra of each fluorophore strongly overlaps the others. Tissue section stained with Alexa 555, 569 and 594; all excited simultaneously at 488 nm. The distribution of each fluorophore is clearly segmented**.
Key Functions
  • Spectral range 365 to 920 nm in a single shot
  • Spectral "unmixing" with auto-spectral libraries
  • Characterize CFP/YFP FRET with over 300 wavelength data points!
  • Conduct pure spectral analysis (absorption, % reflection, %transmission) on micro samples
  • Generate spectral histograms for statistical analysis of regions of interest or entire fields
Key applications
  • Segment and delineate up to 15 fluorophores simultaneously
  • Characterize individual nanoparticles for size and shape.
  • Characterize extended fields of nanoparticles using dark-field scatter
  • Characterize nanorods and nanowires
  • FRET and multiplexed fluorophore imaging
  • Advanced segmentation and analysis of bright-field histological samples
  • OLED defect characterization
To play the PARISS Hyperspectral Imaging "animated" presentation Click Here

* "Alexa Fluor(R)" and "Molecular Probes(TM)" are trademarks of Invitrogen/Molecular Probes
** Taken from "Abstract_FRET_FLIM_Spectral Imaging2" Click here for the pdf

 
Address
  
© 1990-2008 LightForm, Inc all rights reserved
Privacy Policy ............. Disclaimers